Product Basal Media
세포배양배지
아지노모도셀리스트코리아의 중국 햄스터 난소(CHO) 세포주용 CELLiST™ BASAL Media는 아지노모도㈜의 아미노산 기술 역량과 30년 이상의 동물세포배양배지 사업 경험을 바탕으로 개발되었습니다. Chemically Defined 성분으로 동물유래성분을 포함하지 않은 고성능 배지를 제조·판매하고 있습니다.
Chinese Hamster Ovary (CHO)세포의 성장과 재조합 단백질 생산을 하는데 최적화된 Chemically Defined Media 입니다. CELLiST™ BASAL Media는 아지노모도의 역량 및 아미노산에 대한 노하우를 바탕으로 개발되었으며, CHO-K1, CHO-S, CHO-DG44, CHO-DXB11 등 다양한 CHO 세포 라인에 적용 가능합니다.
| Model No. | BASAL3 |
|---|---|
| TYPE | Powder |
| Concentration | 27.0g / L |
| With Additives | D-Glucose |
| Without Additives | L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Poloxamer · Insulin and other growth factors |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 36 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
| Model No. | BASAL3P |
|---|---|
| TYPE | Powder |
| Concentration | 28.0g / L |
| With Additives | D-Glucose · Poloxamer |
| Without Additives | L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine· Insulin and other growth factors |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 24 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with CELLiST F7
A Viable cell density
B IgG Titer (Day 14)
Figure 1: Fed-batch process was performed in an Ambr®15 microbioreactor system, using CHO-K1 cell line expressing IgG1 antibody. Feeding in CELLiSTTM group was performed at a concentration of 6% (v/v) on day 3, 5, 7, 9 and 11. ‘Supplier A, B, C, D and E’ represent basal/feed media combinations from major media suppliers. Culture and feeding manners were performed according to each manufacturer’s recommendations.
Fed-batch process was performed in 125 mL Erlenmeyer flasks, Ambr®15 microbioreactor system, and 2L single-use bioreactor system (Sartorius, BioStat® B), using CHO-DG44, CHO-S and CHO-GS cell lines expressing IgG1 antibody. Feeding was performed at a concentration of 6% (v/v) on day 4, 6, 8, 10 and 12.
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