CELLiST
FEED2

Product Feed Media

세포배양배지

아지노모도㈜의 아미노산 기술과 30년이상 동물세포배양배지사업에 대한 경험을 바탕으로 개발된 동물세포배양배지입니다. 최근엔 모두 Chemically Defined 성분으로 동물유래성분을 포함하지 않은 고성능 배지를 제조·판매하고 있습니다.

CELLiST FEED Media

CHO 세포주의 배양 성능을 향상시킬 수 있도록 설계된 고농축 Feed 배지입니다. CELLiST FEED Media는 단백질 품질과 기능을 유지하면서 목표하는 세포 성장과 생산성을 달성할 수 있도록 지원합니다. FEED2는 모든 배지와 함께 사용할 수 있지만, 기본적으로는 CELLiST BASAL Media와 함께 사용하는데 최적화되었습니다.

특징
  • 화학적으로 조성되었으며 동물 유래 성분이 없음
  • 아지노모도의 오랜 아미노산 연구 역량 및 전문 지식을 바탕으로 배합한 고품질의 아미노산들을 포함하고 있음
  • CHO-K1, CHO-GS, CHO-S, CHO-DG44 및 CHO-DXB11 을 포함한 거의 모든 CHO 셀 라인에 적합함
  • 다양한 사용 목적에 적합한 형태로 포장의 변형이 가능

Specifications

Model No. FEED2
TYPE Powder
Concentration 110.0g / L
With Additives None
Without Additives L-Glutamine·D-Glucose·Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 36 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 6% of FEED2 was added on days 4, 6, 8, 10 and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-GS (CHOZN®) cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against six popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with FEED2

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+FEED2 was evaluated against eight popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-S cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 6, 8, and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

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