Product Basal Media
CELL CULTURE MEDIA
Ajinomoto CELLiST Korea’s CELLiST™ BASAL Media for Chinese Hamster Ovary (CHO) cell lines are developed based on Ajinomoto’s strong capabilities in amino acid technology and over 30 years of experience in the cell culture media business. CELLiST™ Media are high-performance, chemically-defined media, free from any animal-derived ingredients.
growth medium provides optimal balance of amino acids and other nutrients to ensure adequate cell growth and maximum productivity of your process. BASAL CHO MX Medium is completely chemically-defined and does not contain any animal or plant-derived components.
| Model No. | BASAL CHO MX |
|---|---|
| TYPE | Powder |
| Concentration | 23.0g / L |
| With Additives | D-Glucose · Poloxamer |
| Without Additives | L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Insulin and other growth factors |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 24 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
Below are cell culture performance results for CELLiST BASAL CHO MX Medium and several other commer- cially-available media. The fed-batch process was performed using ambr15.
Cell Growth
Figure 1. Viable cell density profiles during 14 days of culture.
Protein Production
Figure 2. IgG titer profile. IgG concentration was measured by Cedex Bio HT.
Scalability
As can be seen below, CELLiST BASAL CHO MX Medium is suitable for use from small-scale to larger scale 200L bioreactors.
Figure 3. Viable cell density and IgG titer profiles. 3 kinds of bioreactor were used, which are ambr15, 3L bench-top reactor, and 200L bioreactor.
