Product Feed Media
细胞培养用补料培养基
基于Ajinomoto(株)的氨基酸技术和三十年以上对动物细胞培养基生产领域的经验而开发的动物细胞培养基。 生产和销售以化学定义成分的不含动物源的高性能培养基。
是被设计成可提高CHO细胞株的培养性能的高浓缩补料培养基。 CELLiST™FEED Media在保持蛋白质品质和功能的同时，支持完成所追求的细胞成长和生产效率。 FEED2可以和所有培养基一起使用，但是基本上最适合同CELLiST™ BASAL Media一起使用。
CELLiST™ F7 feed medium employs Ajinomoto Group’s proprietary cysteine-stabilization technology, ensuring high concentrations of cysteine are continuously available for your CHO cells throughout the culture, leading to greatly improved performance. This is achieved by drastically reducing the naturally-occurring oxidation of L-cysteine to cystine dimers, leading to increase in the amounts of available cysteine, while also improving stability and shelf-life.
CELLiST F7
IgG Titer
Above figure shows >20% increase in performance following 50% increase in available cysteine (1x compared to 1.5x)
(ambr15® culture data, CHO-K1 cell line)
Optimization of amino acids formulation through joint development
with A*STAR’s Bioprocess Technology Institute.
Cutting-edge ‘Digital Twin’ and AI technologies were employed for optimizing metabolic flux in key pathways, such as the TCA cycle, leading to significant improvement in specific productivity and culture growth, and reduced levels of toxic by-products.
| Model No. | F7 |
|---|---|
| TYPE | Powder |
| Concentration | 120.0g / L |
| With Additives | None |
| Without Additives | L-Glutamine·D-Glucose·Insulin and other growth factors |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 24 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with F7
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+F7 was compared to five popular commercial media combinations in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.
CHO-GS cell line
Fed-batch culture of CELLiST BASAL3 with F7
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.
CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with F7
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.
