CHO-K1 cell line Fed-batch culture of CELLiST BASAL3 with CELLiST F7
A Viable cell density
B IgG Titer (Day 14)
Figure 1: Fed-batch process was performed in an Ambr®15 microbioreactor system, using CHO-K1 cell line expressing IgG1 antibody.
Feeding in CELLiSTTM group was performed at a concentration of 6% (v/v) on day 3, 5, 7, 9 and 11.
‘Supplier A, B, C, D and E’ represent basal/feed media combinations from major media suppliers.
Culture and feeding manners were performed according to each manufacturer’s recommendations.
Protein Quality Across Different Scales
Fed-batch process was performed in 125 mL Erlenmeyer flasks, Ambr®15 microbioreactor system,
and 2L single-use bioreactor system (Sartorius, BioStat® B), using CHO-DG44, CHO-S and CHO-GS cell lines
expressing IgG1 antibody. Feeding was performed at a concentration of 6% (v/v) on day 4, 6, 8, 10 and 12.
SAMPLE